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Nucific BIO-X4 Review: Is It Safe and Effective? A Detailed Look

Written by Melissa Mitri, MS, RD

Reviewed by Anthony Dugarte, MD 

Last Updated on May 13, 2021

Our Expert Rating:


About This Rating
The average rating of this product is calculated based on the evaluation of the following factors:
  • Support for Claims:2.0
  • Ingredient Safety:3.0
  • Value for the Price:2.0
  • Brand Transparency:4.0

BIO-X4 is a dietary supplement designed to support smoother digestion, healthy metabolism, and weight loss. It may have the potential to ease digestive symptoms but there is not enough research to support its weight loss and appetite suppression claims.

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Nucific BIO-X4 Review: Is It Safe and Effective? A Detailed Look
Photo by WellnessVerge


  • Some ingredients are evidence-based
  • May have some gut health benefits
  • Gluten, lactose, and allergen-free
  • 90-Day money-back guarantee


  • Limited evidence for weight loss ingredients
  • Potential safety concerns of EGCG green tea extract
  • Expensive for a 30-day supply
  • Digestive enzymes are not necessary for most people

What Is Nucific BIO-X4?

Nucific BIO-X4 is a dietary supplement designed to support optimal digestion and a healthy metabolism.

This product claims that it provides a powerful 4-way defensive strategy against appetite, cravings, digestion, and metabolism.

Dr. Amy Lee is the founder of Nucific, an internal medicine doctor trained in obesity medicine.

The company’s stated mission is to offer cutting-edge products backed by science that allows your body to operate the way it was designed to.

Yet, some of the proposed benefits of this supplement are unsupported by science.

The supplement contains several probiotic strains, digestive enzymes, and ingredients that the manufacturer claims encourage weight loss. Probiotics are beneficial bacteria that may support digestive health.

Additionally, there are claims that due to the digestive enzymes and other ingredients present, this supplement may help improve your metabolism, resulting in weight loss and reduced cravings.

The recommended instructions are to take 3 capsules of Nucific BIO-X4 per day, with 1 capsule at each meal.  Up to 2 capsules per meal can be taken, but exceeding 6 capsules per day is not recommended.

The manufacturers report that the biggest benefits are seen in those who are also following a healthy lifestyle.

In my professional experience working with clients as a Registered Dietitian, I have seen consistently that one food or supplement alone will not lead to dramatic results.

It is the combination of a balanced diet, regular exercise, stress reduction, and sleep quality that more effectively leads to improved digestion and a healthy weight.

Evaluation of Ingredients

Although some of the product claims are questionable, there are some ingredients present in BIO-X4 that are supported by research.

The Lactobacillus and Bifidobacterium probiotic blends combined contain 4 billion CFU’s (colony-forming units) per capsule. The CFU count reflects the number of bacteria present in a supplement.

Nucific BIO-X4 Supplement Facts
Photo by WellnessVerge

For the recommended minimum dose of 3 capsules per day of Nucific BIO-X4, a minimum total of 12 billion CFU’s would be consumed.

In taking a closer look at all of the product claims, here is what I found regarding their validity:

Lactobacillus Blend

Lactobacillus is a group of beneficial bacteria that can support proper digestion and overall health.

The Lactobacillus probiotic blend contains 3 specific Lactobacillus strains: acidophilus, rhamnosus, and plantarum.

Lactobacillus strains are one of the most widely studied probiotic strains.

Our body naturally contains Lactobacillus bacteria. But, if there is no healthy balance of good strains of bacteria, our health can be affected.

There are some studies that demonstrate certain Lactobacillus strains may help manage digestive issues.

A 2018 research review was done on Lactobacillus rhamnosus, which showed improved digestive symptoms such as constipation and diarrhea.

In addition, a 2020 research review showed that Lactobacillus acidophilus might be effective in managing acute and chronic diarrhea as well as antibiotic-associated diarrhea.

Regarding Lactobacillus plantarum, a 2019 animal study showed promise in its ability to support a healthy digestive tract and microbiome. However, a study in mice may not produce the same results in humans.

The research on Lactobacillus strains is strongest related to digestive health, particularly in the case of antibiotic-associated diarrhea.

The research is more scarce regarding the other product claims, such as managing “junk” cravings and weight loss. 

Bifidobacterium Blend

Bifidobacterium is one of the major strains of bacteria lining the gastrointestinal tract.

BIO-X4 contains 2 specific Bifidobacterium strains: lactis and animalis.

One small study in 2010 found that Bifidobacterium lactis reduced gastrointestinal symptoms such as constipation.

A 2014 research review found that Bifidobacterium animalis was seen to have a protective effect against diarrhea and in managing antibiotic-associated diarrhea.

While some of the research on these Bifidobacterium strains is promising, it is unclear the ideal CFU amount needed to produce these effects.

Additionally, some of these studies show more of a correlation in their results based on Bifidobacterium blood levels rather than a direct cause-and-effect relationship.

Digestive Enzyme Blend (43 mg)

The 3 digestive enzymes present are amylase, lipase, and bromelain.

Amylase is an enzyme that breaks down carbs, lipase breaks down fat, and bromelain is an enzyme found in pineapple.

Amylase and lipase are found in various foods such as bananas, papayas, mangoes, and avocados.

While both are necessary enzymes, there is likely no need to take either in supplement form unless you have a deficiency.

Bromelain helps the body digest protein and has been studied for various health benefits for respiratory conditions.

Weight Management Blend (100 mg)

The weight management blend contains the green tea extract EGCG.

This compound, in theory, boosts metabolism and burns body fat. However, the research in this area is mixed.

A 2013 research review concluded that while some studies have shown EGCG to improve fat metabolism, others have not.

In some of the studies reviewed, there were positive results seen in fat-burning potential in those taking at least 270 mg of EGCG per day.

With a dose of 100 mg EGCG per one Nucific BIO-X4 capsule and taking three capsules per day, that would equate to 300 mg of EGCG per day.

This may be enough to produce an effect in a single green tea extract supplement; however, it is questionable if it would produce the same effect as part of a supplement complex like this product.

Craving Control Blend (166 mg)

BIO-X4 contains a “craving control” blend called “slimaluma.”

The ingredient’s actual name is Caralluma fimbriata. It is extracted from a cactus native to India. There are theories that this extract suppresses appetite.

However, this effect has only been seen in children with a specific appetite disorder called Prader-Willi syndrome.

While well-tolerated, a 2015 randomized study was done on Caralluma fimbriata, which showed no benefit in taking it.

For this reason, I would not recommend this ingredient to the general population until more convincing research is out there.

Verdict on Claimed Benefits

Below is our summary of the available evidence for the claimed benefits of Nucific BIO-X4 based on the available research:

  • Supports smoother digestionModerate Evidence
  • Boosts metabolismLimited Evidence
  • Promotes weight managementLimited Evidence
  • Manages cravingsNo Evidence

BIO-X4 Side Effects and Safety

There are some safety concerns regarding a few of the key ingredients in BIO-X4.

This product contains probiotics, which are generally recognized as safe.

However, a 2019 research review showed the potential risk of side effects from probiotics such as gas, bloating, or diarrhea, especially when first taking them.

This research review also found that those who are immunocompromised may also be at a higher risk of infection or sepsis when taking probiotics. 

The side effects of probiotics can vary based on the specific probiotic strain and the health of the individual. Because of this, multiple factors need to be taken into account when determining if it is safe to take.

This supplement also contains EGCG green tea extract. A safe dose of this ingredient has not yet been established.

There have also been rare case reports of liver injury from consuming green tea extracts in doses of 800 mg or more per day. While this is concerning, the dose present in Nucifico BIO-X4 is much lower than this.

Because of the potential for side effects, always consult with your medical provider prior to starting on this or any other supplement.

Nucific BIO-X4 capsules
Photo by WellnessVerge

Cost and Where to Buy

The best place to purchase BIO-X4 is directly from Nucific’s website. A 30-day supply costs $99.00.

This seems expensive compared to other combined probiotic and weight loss products that typically run around $20–$40 for a 30-day supply.

The price is discounted if you order in bulk for a 3-bottle or 6-bottle supply that lasts 3–6 months.

Nucific does offer a full money-back guarantee if you are not satisfied with your results after 90-days.

Although it is sold on Amazon for less, it appears to be sold by third parties. Therefore, there is no guarantee that what is on the label is actually inside the bottle.

How BIO-X4 Compares to Alternatives

Most other digestive supplements contain a blend of both prebiotics and probiotics to help improve gut health – BIO-X4 only contains probiotics.

Ideally, digestive health supplements should contain prebiotics as they help feed the probiotics in the product.

The company claims BIO-X4 is different in that it contains digestive enzymes as well as the craving control ingredient “slimaluma.”

However, most people make enough digestive enzymes on their own; therefore, they don’t need a supplement.

Additionally, the ingredient slimaluma has not shown to be effective.

Dr. Lee claims Nucific’s products are “backed by science.” But the science is not strong enough that a combination of these ingredients is the optimal blend for digestive and weight-related issues.

Related: Best Probiotics of 2021, According to Dietitians

The Bottom Line

The ingredients in BIO-X4 do not have enough evidence to support the weight loss and appetite suppression claims – namely, the green tea extract and slimaluma.

It is also not necessary for most people to take digestive enzymes unless there is a true enzyme deficiency.

However, due to its probiotic content and some research on the benefits of lactobacillus and bifidobacterium strains, using this product may have the potential to ease digestive symptoms.

If you are thinking about taking this supplement, be sure to consult with your medical provider first to outweigh the pros and cons.

A Word from Our RD

As a Registered Dietitian, I would suggest first getting your probiotics from fermented foods like yogurt, kimchi, or kombucha rather than starting with a supplement.

I would also encourage a diet rich in prebiotic fibers that can support the growth of beneficial bacteria. Prebiotics are found in a variety of fruits and vegetables.

Following these diet recommendations and engaging in regular exercise is most effective for achieving optimal digestion and a healthy weight.

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