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Bio-X4 Review: Promising, But Requires Consistency and Time

By Anastasia Climan, RDN, CD-N

Last Updated on May 8, 2023

Medically Reviewed by Natalie Olsen, MS, RDN

Dietitian Rating:


About This Rating
The average rating of this product is calculated based on the evaluation of the following factors:
  • Support for Claims:4.0
  • Ingredient Safety:4.5
  • Value for the Price:4.0
  • Customer Service:5.0

Bio-X4 is a dietary supplement designed for digestion and metabolism that may be worth trying if you have issues like gas, bloating, and unhealthy cravings. The supplement contains research-backed ingredients, but you must give it a few months to see how you respond.

Written by
Anastasia Climan, RDN, CD-N
Registered Dietitian Nutritionist
Anastasia Climan is a registered dietitian whose career progressed into public health, corporate wellness, and private practice roles. She earned a Bachelor of Science from the University of Connecticut's Coordinated Undergraduate Dietetics Program in 2011. She's led wellness initiatives on HIV/AIDS, children's health, and diabetes.
Medically Reviewed by
Natalie Olsen, MS, RDN, LD, ACSM-EP
Registered Dietitian, Certified Exercise Physiologist
Natalie is a registered dietitian, functional medicine practitioner and certified exercise physiologist with over 15 years experience in the health and wellness industry and holds a Master's degree in Human Nutrition and Functional Medicine through a collaborative program provided by the University of Western States and the Institute of Functional Medicine.
Bio-X4 Review: Promising, But Requires Consistency and Time
Photo by WellnessVerge

Key Takeaways (TL;DR)

  • Bio-X4 contains well-researched ingredients that may benefit digestion, especially when taken alongside a healthy diet.
  • This supplement is for anyone who struggles with bloating, diarrhea, constipation, and cravings for unhealthy food.
  • Bio-X4 may not work immediately. You will need to give it a couple of months to experience its benefits.
  • Overall, customers seem happy with the product and say it helps them stay regular, reduces bloating, and helps to handle unhealthy food cravings better. However, not too many people report significant weight loss.
  • The ingredients in Bio-X4 are unlikely to cause significant side effects, but you may notice some digestive changes when you first start taking it.

What Bio-X4 Gets Right

  • It uses ingredients that have established benefits for digestion and metabolism.
  • The product is third-party tested for quality and purity.
  • The brand has a 90-day refund guarantee that allows you to test the product with little risk.
  • The brand encourages taking Bio-X4 alongside a healthy diet to experience the most benefit.

Important Considerations

  • You need to take the product three times a day, which may take some people time to adjust to.
  • It might take several months before you notice any benefits.
  • Ongoing monthly cost should be considered to make sure it fits your budget.

Bio-X4 at a Glance

Bio-X4 from Nucific is a dietary supplement designed to support various areas of the digestive system and metabolism.

It contains digestive enzymes to break down food, probiotics to improve digestion and bowel movements, and other ingredients, including green tea extract and Slimaluma, that may speed up the metabolism and prevent fat gain.

Taking Bio-X4 with each meal is designed to change how your body digests food for the better. Previous “trouble foods” can pass through your system more efficiently, so you can have regular bowel movements and fewer unpleasant symptoms like gas and bloating.

Expand to See Supplement Facts

  • Probiotic Blend, 4 billion CFU: L. acidophilus, L. rhamnosus, L. plantarum, B. lactis, and B. animalis
  • Digestive Enzyme Blend, 43 mg: Amylase, Bromelain, and Lipase
  • Weight Management Blend, 100 mg: Green Tea Extract (50% EGCG)
  • Cravings Control Blend, 166 mg: Slimaluma (Caralluma fimbriata extract)
  • Inactive Ingredients: Vegetable cellulose, Microcrystalline cellulose, Stearic acid, and Silica

How Do I Take This Supplement?

The recommended dose of Bio-X4 is one capsule, three times per day, with meals. However, you can increase your intake to two capsules per meal, not exceeding six daily capsules.

Since it typically takes three months of regular use to witness the full benefits of Bio-X4, it's recommended to begin with a 90-day supply. Additionally, most research shows that it generally takes 2–6 weeks to start seeing results from taking probiotics.

Subscription Is Cost-Effective for Regular Use + Good Money-Back Guarantee

One-Time Purchase


1 Bottle (1-Month supply)



3 Bottles (3-Month supply)

$129 ($43 per bottle)

$114 ($38 per bottle)

6 Bottles (6-Month supply)

$240 ($40 per bottle)

$216 ($36 per bottle)

Bio-X4 is only available through Nucific’s website. You can’t purchase it in stores or on third-party sites. Shipping is free in the US for any purchases over $50.

If you’re unhappy with Bio-X4, you can return it for a full refund within 90 days. The terms state that even if you use up all of the product, you will get your money back if you return the empty bottle(s).

Try Bio-X4

Key Benefits of Bio-X4

Bio-X4 is designed to deliver the following health benefits:

  • Manage cravings
  • Weight management
  • Digestive support
  • Boosts metabolism

The formulation of Bio-X4 has not been tested as a whole. However, the ingredients that it contains have been individually studied. Below we compare this research against the product’s claimed benefits.

Manage Cravings

Slimaluma, a patented extract made from the popular medicinal plant Caralluma fimbriata, is traditionally known as an appetite suppressant. Some studies show that Slimaluma may help reduce unhealthy food cravings and control appetite. (1, 2, 3, 4)

Weight Management

Caralluma fimbriata and green tea extract (The green tea extract used in Bio-X4 is 50% EGCG) have studies showing that these ingredients help promote healthy weight management and reduce waist circumference. Researchers concluded that the weight loss results were likely caused by Caralluma fimbriata’s effect on controlling appetite. (3)

Green tea extract improves the body’s ability to break down body fat during steady-state exercise. Studies have shown that supplementing green tea extract leads to significant weight loss and other health benefits, such as lowering cholesterol. (5, 6, 7)

Finally, one of EGCG’s benefits includes its “anti-obesity” effects, as it increases hormones related to fat metabolism. (8)

Digestive Support

The digestive enzymes and probiotics in Bio-X4 are responsible for its digestive health claims.

Digestive enzymes indeed help break down food. However, most healthy people produce enough digestive enzymes and don’t require a supplement.

Enzymes are essential for optimal digestion, and our bodies naturally produce various enzymes, including lipase and amylase. (9) There are many different enzymes, but Bio-X4 includes amylase, bromelain, and lipase, which help break down carbohydrates, proteins, and fats.

Additionally, research shows that probiotics are generally beneficial for digestive symptoms such as diarrhea and constipation and may help with irritable bowel disease and bloating. (10) Specifically, Bio-X4 contains Lactobacillus and Bifidobacterium, two of the most well-researched probiotics. (11, 12, 13)

Boosts Metabolism

There’s evidence that green tea extract has metabolism-boosting effects, promoting fat breakdown and stimulating fat-burning by producing body heat. (14, 7)

There are no clear dosing guidelines for EGCG (Bio-X4's green tea extract). Still, studies show a wide range of doses between 100–856 milligrams for boosting metabolism. The amount of EGCG in Bio-X4 should be sufficient to offer benefits. A day’s worth of Bio-X4 contains 150–300 milligrams of EGCG (depending if you take one or two pills with each meal). (14, 6)

Nucific BIO-X4 capsules
Photo by WellnessVerge

Bio-X4 Is Generally Safe for Most People

Bio-X4 is a generally safe supplement unlikely to cause severe or lasting side effects.

The probiotic strains found in Bio-X4 are unlikely to cause harm for healthy people. (15) However, if you are pregnant or breastfeeding or have a compromised immune system or other underlying conditions, you could be at risk of harmful effects from probiotics. (16)

Additionally, although the green tea extract used in this supplement is in safe amounts, it may interfere with certain medications. (17)

Because digestive enzymes and probiotics help your body digest food more efficiently, you may head to the bathroom more frequently.

Regarding Slimaluma, a review of several studies using the herbal extract found in this ingredient reported the main side effects as constipation, diarrhea, nausea, and rashes. (1)

Always speak with your healthcare provider before taking supplements like Bio-X4.

How Bio-X4 Compares to Alternatives

At its core, Bio-X4 is a probiotic supplement that includes digestive enzymes and several additional metabolism-boosting ingredients. Its ingredients are also third-party tested for quality and purity. These factors make Bio-X4 unique.

You can find probiotic and digestive enzyme blends for less money than Bio-X4, but they don’t have additional ingredients that Bio-X4 contains, and their probiotic content is lower. However, the other brands offer more variety and amounts of digestive enzymes.

Here’s how Bio-X4 compares to two similar products from well-known brands that combine probiotics and digestive enzymes.

Nucific Bio-X4

Life Extension Enhanced Super Digestive Enzymes and Probiotics

Enzymedica Digest Gold Probiotics

Probiotic Strains and Count

5 (4 billion CFU)

1 (1 billion CFU)

9 (500 million CFU)

Digestive Enzymes




Other Ingredients

Green Tea Extract, and Camulluna Fibriata

Prebiotics (food for probiotics)


Claimed Benefits

Improves digestion, Raises Metabolism

Improves digestion, Promotes nutrient absorption

Improves digestion, Supports immunity and energy levels

Third-Party Tested



Form & Daily Dosage

3 capsules

1–2 capsules

3 capsules

Allergen and Special Diet Considerations

Allergen-free, Gluten-free

Vegetarian, Non-GMO

Vegetarian, Dairy-free, Gluten-free, Non-GMO, Soy-free





Money-Back Guarantee

90 days

12 months

30 days

Tip: If Bio-X4 is outside your budget, consider a regular probiotic supplement, which is usually cheaper. Use our list of the best probiotics to find the right one for you.

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At WellnessVerge, we only use reputable sources, including peer-reviewed medical journals and well-respected academic institutions.

  1. The use of Caralluma fimbriata as an appetite suppressant and weight loss supplement: a systematic review and meta-analysis of clinical trials - PubMed:
  2. Effect of Caralluma fimbriata extract on appetite, food intake and anthropometry in adult Indian men and women - PubMed:
  3. The effect of an orally-dosed Caralluma Fimbriata extract on appetite control and body composition in overweight adults - PMC:
  4. Delving the Role of Caralluma fimbriata: An Edible Wild Plant to Mitigate the Biomarkers of Metabolic Syndrome - PubMed:
  5. Nutrients | Free Full-Text | The Impact of Decaffeinated Green Tea Extract on Fat Oxidation, Body Composition and Cardio-Metabolic Health in Overweight, Recreationally Active Individuals | HTML:
  6. Therapeutic effect of high-dose green tea extract on weight reduction: A randomized, double-blind, placebo-controlled clinical trial - PubMed:
  7. [Effects of green tea and its epigallocatechin (EGCG) content on body weight and fat mass in humans: a systematic review] - PubMed:
  8. Effects of green tea extract on overweight and obese women with high levels of low density-lipoprotein-cholesterol (LDL-C): a randomised, double-blind, and cross-over placebo-controlled clinical trial - PubMed:
  9. Digestive Enzymes and Digestive Enzyme Supplements | Johns Hopkins Medicine:
  10. Probiotics and Gastrointestinal Conditions: An Overview of Evidence from the Cochrane Collaboration - PMC:
  11. Lactobacillus spp. for Gastrointestinal Health: Current and Future Perspectives - PMC:
  12. Probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium lactis Bi-07 versus placebo for the symptoms of bloating in patients with functional bowel disorders: a double-blind study - PubMed:
  13. Effect of combined bifidobacteria supplementation and resistance training on cognitive function, body composition and bowel habits of healthy elderly subjects - PubMed:
  14. The Anti-Obesity and Health-Promoting Effects of Tea and Coffee - PMC:
  15. Probiotics - Health Professional Fact Sheet:
  16. Probiotics: What You Need To Know – NIH:
  17. Drug Interactions between green tea and simvastatin – Drugs.com: