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Nucific BIO-X4 Review: Is It Safe and Effective? A Detailed Look

By Melissa Mitri, MS, RD

Medically Reviewed by Anthony Dugarte, MD

Last Updated on November 5, 2021

Dietitian Rating:


About This Rating
The average rating of this product is calculated based on the evaluation of the following factors:
  • Support for Claims:3.0
  • Ingredient Safety:3.0
  • Value for the Price:2.0
  • Brand Transparency:4.0

BIO-X4 is a dietary supplement designed to support smoother digestion, a healthy metabolism, and weight loss. This product may support your digestive tract, but the research on its weight loss claims is not convincing enough.

Written by
Melissa Mitri, MS, RD
Registered Dietitian Nutritionist
Melissa Mitri, MS, RD, is a registered dietitian nutritionist, weight loss specialist, and expert health writer with over 15 years of experience in the health and wellness industry. Melissa has worked in a variety of healthcare settings helping clients improve their diet through an individualized approach.
Medically Reviewed by
Anthony Dugarte, MD
Medical Reviewer
Anthony Dugarte, M.D., C.S.C.S. is a health and wellness writer and medical content reviewer. In addition to dedicating the last 7 years to medical research, Dr. Dugarte also has more than a decade of experience in strength and conditioning, nutrition, and rehabilitative exercise, as well as authoring and reviewing health and wellness-related content.

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Nucific BIO-X4 Review: Is It Safe and Effective? A Detailed Look
Photo by WellnessVerge


  • Some ingredients are evidence-based
  • May support gut health
  • Gluten, lactose, and allergen-free
  • 90-Day money-back guarantee


  • Lack of convincing research on weight loss benefits
  • Expensive for a 30-day supply
  • Digestive enzymes are not necessary for most people

What Is Nucific BIO-X4?

Nucific BIO-X4 is a dietary supplement designed to support optimal digestion and a healthy metabolism.

This product claims that it provides a powerful 4-way defensive strategy against a big appetite, food cravings, indigestion, and poor metabolism.

Dr. Amy Lee is the founder of Nucific, an internal medicine doctor trained in obesity medicine.

The company’s stated mission is to offer cutting-edge products backed by science that allow your body to operate the way it was designed to.

BIO-X4 contains several probiotics, digestive enzymes, and other ingredients that the manufacturer claims encourage weight loss.

The four central health claims of BIO-X4 are:

  • It manages “junk” cravings
  • It promotes weight management
  • It supports smoother digestion
  • It boosts metabolism

Evaluation of Ingredients

BIO-X4 contains the ingredient blends discussed below that are intended to produce its desired effects. The ingredient amounts listed below are for one capsule only.

Dosage recommendations are to take at least one capsule per meal and up to 6 capsules per day.

The Probiotic and Digestive Enzyme blends contain multiple ingredients as part of the blend. Therefore, the individual ingredient doses in these two blends are unknown.

Nucific BIO-X4 Supplement Facts
Photo by WellnessVerge

Probiotic Blend, 4 Billion CFUs

The Probiotic Blend is made up of two bacterial blends: Lactobacillus Blend and Bifidobacterium Blend.

Lactobacillus Blend

Lactobacillus is a group of beneficial bacteria that can support proper digestion and overall health.

The Lactobacillus Blend contains three specific strains: L. acidophilus, L. rhamnosus, and L. plantarum.

Lactobacillus strains are one of the most widely studied probiotic strains.

Our body naturally contains a certain amount of lactobacillus bacteria. But, if there are more bad versus good strains of bacteria present, our health can be affected. (1)

Some studies show that certain Lactobacillus strains may help manage digestive issues.

For example, a 2018 research review was done on L. rhamnosus, which showed improved digestive symptoms such as constipation and diarrhea. (2)

In addition, a 2020 research review showed that L. acidophilus might be effective in managing acute and chronic diarrhea as well as antibiotic-associated diarrhea. (3)

Regarding L. plantarum, a 2019 animal study showed promise in its ability to support a healthy digestive tract and microbiome. However, a study in mice may not produce the same results in humans. (4)


The research on the Lactobacillus strains present in this product is strongest related to digestive health, particularly in the case of antibiotic-associated diarrhea.

The research is scarcer regarding the other product claims, such as managing “junk” cravings and producing weight loss.

Bifidobacterium Blend

Bifidobacterium is one of the major strains of bacteria lining the gastrointestinal tract.

BIO-X4 contains two specific strains: Bifidobacterium lactis and Bifidobacterium animalis.

One small study in 2010 found that B. lactis reduced gastrointestinal symptoms such as constipation. (5)

Another 2014 research review found that B. animalis was seen to have a protective effect against diarrhea and, in particular, managing antibiotic-associated diarrhea. (6)


Some research on the Bifidobacterium strains in BIO-X4 is promising, but it is unclear what the ideal CFU amount is needed to produce these effects.

Additionally, some of these studies show more of a connection between this strain and digestive problems rather than a direct cause-and-effect relationship from taking this probiotic in supplement form.

Digestive Enzyme Blend, 43 mg

The three digestive enzymes present in this blend are amylase, lipase, and bromelain.

Amylase is an enzyme that breaks down carbs, and lipase breaks down fat.

Amylase and lipase are found in various foods such as bananas, papayas, mangoes, and avocados.

While both are necessary enzymes, there is likely no need to take either in supplement form unless you have a deficiency.

Bromelain helps the body digest protein and has been studied for various health benefits, such as respiratory conditions and diarrhea. Bromelain is found in pineapples.  (7)

A 2012 research review on bromelain showed it has the potential to reduce diarrhea in animals caused by infections such as E. coli(8)

However, human trials on bromelain’s role in improving digestion without an infection present are lacking.


Unless you have a diagnosed digestive enzyme deficiency, it is unclear if taking this blend will provide any benefit.

Weight Management Blend, 100 mg

The weight management blend consists of green tea extract (GTE) as its main ingredient. The extract contains 50% EGCG, a plant compound present in green tea leaves thought to be responsible for many of its touted health benefits.

EGCG is said to boost metabolism and burn body fat. However, the research in this area is mixed.

A 2013 research review concluded that while some studies have shown EGCG to improve fat metabolism, others have not. (9)

In some of the studies reviewed, positive results were seen regarding its fat-burning potential in those taking at least 270 mg of EGCG per day.

With a dose of 50 mg EGCG per one Nucific BIO-X4 capsule and taking three capsules per day, that would equate to 150 mg EGCG per day.

To maximize the fat-burning potential of this supplement, you can take up to 6 capsules per day, according to the manufacturer, for a total of 300 mg EGCG.


Green tea extract and its main compound, EGCG, have been seen to support weight loss in several studies. However, most of the studies to date are smaller in size, and therefore more extensive studies would be helpful to determine the benefits for the general population.

Craving Control Blend, 166 mg

BIO-X4 contains a “craving control” blend called Slimaluma.

The ingredient’s actual scientific name is Caralluma fimbriata, abbreviated as CFE. It is extracted from a type of cactus native to India. There are theories that this extract suppresses appetite.

While well-tolerated, a 2015 randomized study was done on CFE, which showed no benefit in taking it. (10)

A more recent 2021 randomized controlled trial demonstrated more of an effect. (11)

In this 4-month study, the CFE group consumed around 245 fewer calories per day on average. They also lost more weight and saw a reduction in their waist circumference.

While these results show more promise, the CFE group lost less than 1 pound on average and only about an inch off their waistlines. This is not an impressive amount of weight to lose in four months.

The ideal dose of CFE to produce benefits is also still being investigated.


Caralluma fimbriata is a plant extract promoted to reduce appetite and support weight loss. There is some potential for its use, but study results thus far are not convincing enough to show it has a significant impact on weight.

Verdict on Claimed Benefits

Below is our summary of the available evidence for the claimed benefits of Nucific BIO-X4 based on the available research:

Manages “junk” cravingsModerate Evidence
Promotes weight managementModerate Evidence
Supports smoother digestionModerate Evidence
Boosts metabolismModerate Evidence

There is some research to support some of the claims made for this product, such as its potential to reduce weight, decrease appetite, and lessen digestive symptoms and diarrhea in the case of an infection.

However, additional larger studies are needed on green tea extract and CFE to determine how much of an effect on weight loss they actually have.

For someone who has indigestion that is not related to an infection, it’s unclear how much of a benefit taking these particular probiotics will have.

Side Effects, Safety

Dosage instructions are to take one capsule of BIO-X4 with each meal. Up to 2 capsules per meal can be taken, but not to exceed six capsules per day.

There are a few potential side effects to note from taking this product.

BIO-X4 contains probiotics, which are generally recognized as safe.

However, a 2019 research review showed the potential risk of side effects from probiotics such as gas, bloating, or diarrhea, especially when first taking them. (12)

This research review also found that those who are immunocompromised may also be at a higher risk of infection or sepsis when taking probiotics.

The side effects of probiotics can vary based on the specific probiotic strain and the individual’s health. Because of this, multiple factors need to be taken into account when determining if it is safe to take.

This supplement also contains EGCG green tea extract. There have been rare case reports of liver injury from consuming EGCG in doses of 800 mg or more per day. (13)

While this is concerning, the EGCG dose present in Nucific BIO-X4 is much lower than this.

Additionally, a 2018 systematic review determined that up to 338 mg of EGCG in capsule form is considered safe with minimal risk for side effects. (14)

Based on the amount present in one capsule of BIO-X4, the highest amount of EGCG you would be consuming would be 300 mg per day from 6 capsules.

Always consult with your medical provider before starting on this or any other supplement.

Nucific BIO-X4 capsules
Photo by WellnessVerge

Cost and Where to Buy

The best place to purchase BIO-X4 is directly from Nucific’s website. A 30-day supply costs $99.00.

This seems expensive compared to other weight loss probiotic supplements that typically run around $20–$40 for a 30-day supply.

The price is discounted if you order in bulk for a 3-bottle or 6-bottle supply that lasts 3–6 months.

Nucific does offer a full money-back guarantee if you are not satisfied with your results after 90-days.

Although it is sold on Amazon for less, it appears to be sold by third parties. Therefore, there is no guarantee that what is on the label is actually inside the bottle.

How BIO-X4 Compares to Alternatives

There are a few products on the market with similar digestive health and weight management claims as Nucific’s BIO-X4, but not many with the same combination of ingredients to produce this intended benefit.

The two most similar products in their health claims and ingredients are New Chapter Weight Management Probiotics and Prebiotics and HUM Nutrition’s Skinny Bird.

New Chapter’s product also contains a combination of digestive and weight management ingredients.

It contains only the bacterial strain B. animalis but at a higher potency. However, BIO-X4 contains several strains that may work better together.

New Chapter contains inulin, a prebiotic that may support the absorption of the probiotics present.

New Chapter also contains green coffee bean extract. This is different from the green tea extract present in BIO-X4 but has similar claims and efficacy concerning weight management.

Hum Nutrition’s Skinny Bird does not contain any probiotics, but like BIO-X4, it contains Caralluma fimbriata (CFE) and green tea extract (GTE) for weight management.

The CFE present is twice the amount of BIO-X4, but the GTE dose is lower.

Skinny Bird also contains an additional ingredient called 5-HTP, which has been seen to increase satiety and reduce BMI in some studies. (15)

HUM Nutrition is a supplement company that utilizes quizzes to make more personalized supplement recommendations.

HUM Nutrition employs registered dietitians to answer your questions and point you in the right direction for the best supplement for your needs.

HUM is also more transparent in sharing relevant research studies on their website, which adds to their credibility.

For a price comparison, HUM is the best price at $40 per month, New Chapter’s blend is $52.63 per month, and BIO-X4 is the most expensive at $99.00 per month.

If you are solely looking for a weight loss supplement, I would suggest Hum’s Skinny Bird out of these three.

If you need more digestive support, you can take a single probiotic supplement or consume fermented foods like yogurt and kefir.

Related: Best Probiotics of 2022, According to Dietitians

The Bottom Line

Based on the available research, Nucific’s BIO-X4 is generally seen as safe with minimal side effects. However, larger studies on its ingredients are needed to confirm its effectiveness.

Based on some of the ingredients present in BIO-X4, it’s possible you may lose a small amount of weight.

Although based on the available studies to date, it likely won’t be significantly different than something you were doing on your own.

If you are experiencing infection or antibiotic-associated diarrhea, the probiotics in this product may help lessen these symptoms.

If you are thinking about taking this or any new supplement, be sure to consult with your medical provider to outweigh the pros and cons.

Next Steps

The manufacturers report that the most significant benefits from BIO-X4 are seen in those who follow a healthy lifestyle. I can attest to this statement from my experience as a registered dietitian.

In working with clients, I have consistently seen that no one food or supplement alone will lead to dramatic results.

Instead, it is the combination of a balanced diet, regular exercise, stress reduction, and sleep quality that more effectively leads to improved digestion and a healthy weight.

If you are struggling with indigestion or are trying to lose weight, I would suggest first getting your probiotics from fermented foods like yogurt, kimchi, or kombucha rather than starting with a supplement.

These foods are available in most grocery stores and can be easily added to your daily routine.

I would also encourage a diet rich in prebiotic fibers that can support the growth of beneficial bacteria and also help to keep you full to support an optimal weight.

Prebiotics are found in a variety of fruits and vegetables.

Following these diet recommendations and engaging in regular exercise is most effective for achieving optimal digestion and a healthy weight.

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At WellnessVerge, we only use primary references for our articles, including peer reviewed medical journals or well-respected academic institutions.

  1. The intestinal microbiota in health and disease:
  2. Probiotic research priorities for the healthy adult population: A review on the health benefits of Lactobacillus rhamnosus GG and Bifidobacterium animalis subspecies lactis BB-12:
  3. Lactobacillus acidophilus LB: a useful pharmabiotic for the treatment of digestive disorders:
  4. The Impact of Lactobacillus plantarum on the Gut Microbiota of Mice with DSS-Induced Colitis:
  5. Dose-response effect of Bifidobacterium lactis HN019 on whole gut transit time and functional gastrointestinal symptoms in adults:
  6. The Science behind the Probiotic Strain Bifidobacterium animalis subsp. lactis BB-12®:
  7. Properties and Therapeutic Properties of Bromelain: A Review:
  8. Properties and Therapeutic Application of Bromelain: A Review:
  9. The Effect of Green Tea Extract on Fat Oxidation at Rest and during Exercise: Evidence of Efficacy and Proposed Mechanisms:
  10. To evaluate efficacy and safety of Caralluma fimbriata in overweight and obese patients: A randomized, single blinded, placebo control trial:
  11. The effect of an orally-dosed Caralluma Fimbriata extract on appetite control and body composition in overweight adults:
  12. Probiotics: If It Does Not Help It Does Not Do Any Harm. Really?:
  13. Scientific opinion on the safety of green tea catechins:
  14. The safety of green tea and green tea extract consumption in adults – Results of a systematic review:
  15. Relationship between the absorption of 5-hydroxytryptophan from an integrated diet, by means of Griffonia simplicifolia extract, and the effect on satiety in overweight females after oral spray administration: